Chemistry 542

Chromatographic Methods

Spring, 2001

Instructor: Dr. Neil Brotherton                                               Office: CNSB 111

E-mail: hbrotherton@spock.ulm.edu                                            Phone: 1829 

Office hours: MWF (10:00 - 10:50)

Lecture: CNSB 245, MWF (9:00-9:50)

Prerequisite: CHEM 407 ("C" or better)

Lecture Text: Principles and Practice of Modern Chromatographic Methods , K. Robards, P. R. Haddad, P. E. Jackson. Academic Press.

Course Objective: This course will take students through the basic theoretical concepts behind chemical separations and will continue with an in depth discussion of planar, packed column, and open column chromatographic separations. Students will gain an understanding of the instrumentation and equipment necessary to accomplish the separations and the ability to evaluate the data which is obtained. The basics of column and mobile phase selection as well as the fundamentals of instrument design will also be discussed.

Grading: There will be three tests plus a final examination. Dates are to be determined. Each test will be graded on a basis of 100 points. Each student will be required to prepare a paper on a selected area of modern chromatography for up to 100 points. This provides for a maximum of 500 points. At the end of the semester, all grades will be assigned based on the following percentages:

90-100 A 80-90 B 70-80 C 60-70 D 0-59 F

 

Attendance is strongly encouraged in the lecture and based on University policy.

Absences from tests: If the absence is unexcused, the grade on the examination is a zero. If the absence is excused, you will be administered a makeup test at the end of the semester. Excuses for missed examinations must be submitted to the instructor within one week of the examination date unless you are physically unable to do so.

 

 

Lecture Outline

I. Introduction

II. Separation Theory

III. Planar Chromatography 

IV. Gas Chromatography   

          A. Instrumentation 

          B. Detectors    

          C. Columns: packed, capillary, and megabore (widebore)

V. Liquid Chromatography  

          A. Instrumentation 

          B. Detectors  

          C. Ion Exchange  

          D. Size Exclusion

VI. Supercritical Fluid Chromatography  

          A. Instrumentation 

          B. Columns